This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Thy-1-CRISPR-gRNA#3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)
catalog :
124285
product information
Catalog Number :
124285
Product Name :
Thy-1-CRISPR-gRNA#3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)
article :
doi
id28197548
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepSpCas9(BB)-2A-Puro (PX459) V2.0
backbone_mutation
backbone_originFeng Zhang (Addgene plasmid # 62988)
backbone_size9200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth notes :
Plus G was routinely added because U6 promoter prefers G as the first transcribed nucleotide. gRNA sequence contains at least 3 mismatches, and was selected by choosing the highest scored sequence from different programs and databases.
growth strain :
sgRNA against human Thy-1
origin :
37
pi :
alt_names
CD90
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoter
sequencing_primer_3
sequencing_primer_5U6-fw: GGGCCTATTTCCCATGATTCCTTC
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesCD90, CDw90
geneTHY1
id7070
genbank_ids
mutation
nameHomo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1
shRNA_sequence(g)TAGCAGGAGAGCGATGCTGA
size21
species
9606
Homo sapiens
tags
resistance markers :
4393
4392
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA