pQE30-His-T7RNAP | Addgene 124138 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pQE30-His-T7RNAP

catalog :

124138

citations: 4

Reference
Ngo P, Ishida S, Busogi B, Do H, Ledesma M, Kar S, et al. Changes in Coding and Efficiency through Modular Modifications to a One Pot PURE System for In Vitro Transcription and Translation. ACS Synth Biol. 2023;12:3771-3777 pubmed publisher
Sobeh A, Eichhorn C. C-terminal determinants for RNA binding motif 7 protein stability and RNA recognition. Biophys Chem. 2023;292:106928 pubmed publisher
Pandi A, Diehl C, Yazdizadeh Kharrazi A, Scholz S, Bobkova E, Faure L, et al. A versatile active learning workflow for optimization of genetic and metabolic networks. Nat Commun. 2022;13:3876 pubmed publisher
Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, et al. Cell-free translation reconstituted with purified components. Nat Biotechnol. 2001;19:751-5 pubmed

product information

Catalog Number :

124138

Product Name :

pQE30-His-T7RNAP

article :

doi	10.1038/90802
id	28197403
pubmed_id	11479568

bacterial resistance :

Ampicillin

cloning :

backbone

pQE30

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

This plasmid is provided in NEB Stable cells from Addgene; however, the depositor originally cloned this plasmid in JM109 cells. It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.

growth strain :

Bacterial expression plasmid for PURE system components

inserts :

JM109

origin :

pi :

alt_names

T7 RNAP

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5	Unknown
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	T7p07
gene	T7p07
id	1261050

genbank_ids

mutation

name

T7 RNA polymerase

shRNA_sequence

size

species

32644

Other

Enterobacteria phage T7