This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET29b-IPP1-His
catalog :
124137
citations: 2
Reference
Tersteeg S, Mrozowich T, Henrickson A, Demeler B, Patel T. Purification and characterization of inorganic pyrophosphatase for in vitro RNA transcription. Biochem Cell Biol. 2022;100:425-436 pubmed publisher
Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, et al. Cell-free translation reconstituted with purified components. Nat Biotechnol. 2001;19:751-5 pubmed
product information
Catalog Number :
124137
Product Name :
pET29b-IPP1-His
article :
doi10.1038/90802
id28197403
pubmed_id11479568
bacterial resistance :
Kanamycin
cloning :
backbonepET29b(+)
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
It is recommended to screen DNA preps from multiple colonies by Sanger sequencing, as this plasmid may be prone to deletions in the promoter region.
growth strain :
Bacterial expression plasmid for PURE system components
inserts :
JM109
origin :
37
pi :
alt_names
IPP1
PPase
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5Unknown
promoter
sequencing_primer_3
sequencing_primer_5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYBR011C, PPA1
geneIPP1
id852296
genbank_ids
mutation
nameInorganic pyrophosphatase
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on insert
tag6x-His
resistance markers :
4217
4380
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA