This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pEW3: pET-32a Dot1L(2-416)
catalog :
124098
citations: 1
Reference
Worden E, Hoffmann N, Hicks C, Wolberger C. Mechanism of Cross-talk between H2B Ubiquitination and H3 Methylation by Dot1L. Cell. 2019;176:1490-1501.e12 pubmed publisher
product information
Catalog Number :
124098
Product Name :
pEW3: pET-32a Dot1L(2-416)
article :
doi10.1016/j.cell.2019.02.002
id28197523
pubmed_id30765112
bacterial resistance :
Ampicillin
cloning :
backbonepET-32a
backbone_mutation
backbone_originNovagen
backbone_size5888
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Dot1L(2-416) expression plasmid
origin :
37
pi :
alt_names
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterT7
sequencing_primer_3T7 Terminator
sequencing_primer_5T7 Promotor
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDOT1, KMT4
geneDOT1L
id84444
genbank_ids
mutation
nameDot1L(2-416)
shRNA_sequence
size1245
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagTrx-His-TEV
plasmid copy :
Addgene plasmid # 74173
resistance markers :
583
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA