This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
HAP40 1-371
catalog :
124060
citations: 2
Reference
Harding R, Deme J, Hevler J, Tamara S, Lemak A, Cantle J, et al. Huntingtin structure is orchestrated by HAP40 and shows a polyglutamine expansion-specific interaction with exon 1. Commun Biol. 2021;4:1374 pubmed publisher
Harding R, Loppnau P, Ackloo S, Lemak A, Hutchinson A, Hunt B, et al. Design and characterization of mutant and wildtype huntingtin proteins produced from a toolkit of scalable eukaryotic expression systems. J Biol Chem. 2019;294:6986-7001 pubmed publisher
product information
Catalog Number :
124060
Product Name :
HAP40 1-371
article :
doi10.1074/jbc.RA118.007204
id28193494
pubmed_id30842263
bacterial resistance :
Ampicillin
cloning :
backbonepFBOH-MHL
backbone_mutation
backbone_originArrowsmith lab (Addgene plasmid# 62304)
backbone_size6767
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Baculovirus expression
growth notes :
Please visit https://www.biorxiv.org/content/early/2018/12/10/492215 for BioRxiv preprint.
growth strain :
Baculovirus expression vector for HAP40 protein (aa 1-371) in insect cells
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterpolyhedrin
sequencing_primer_3TAACCATTATAAGCTGC
sequencing_primer_5AGTATTTTACTGTTTTCG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDXS522E, F8A, HAP40
geneF8A1
id8263
genbank_ids
mutation
nameHAP40
shRNA_sequence
size1113
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag6x His
locationN terminal on backbone
tagTEV cleavage site
resistance markers :
732
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA