This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFS177
catalog :
12380
citations: 1
Reference
Sivakumar S, Porter Goff M, Patel P, Benoit K, Rhind N. In vivo labeling of fission yeast DNA with thymidine and thymidine analogs. Methods. 2004;33:213-9 pubmed
product information
Catalog Number :
12380
Product Name :
pFS177
article :
doi10.1016/j.ymeth.2003.11.016
id860
pubmed_id15157888
bacterial resistance :
Ampicillin
cloning :
backbonepART1
backbone_mutation
backbone_origin
backbone_size7300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
Please note that Addgene's sequencing results identified N81S and M84V mutations in the hENT1 sequence when compared to GenBank entry AAC51103. These mutations were not intentional and this plasmid is exactly as described in the associated article listed below. The depositing laboratory states that the hENT1 in the plasmid is functional.
origin :
37
pi :
alt_names
hENT1
cloning
clone_methodRestriction Enzyme
cloning_site_3SacI
cloning_site_5BamHI
promoter
sequencing_primer_3n/a
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesAUG, ENT1, hENT1
geneSLC29A1
id2030
genbank_ids
AAC51103
mutationN81S and M84V
namehuman equilibrating nucleoside transporter 1
shRNA_sequence
size1400
species
9606
Homo sapiens
tags
plasmid copy :
IMAGE Consortium
resistance markers :
416
tags :
High Copy
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA