pCL-MFG-BRCA1 | Addgene 12341 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCL-MFG-BRCA1

catalog :

12341

citations: 1

Reference
Ruffner H, Verma I. BRCA1 is a cell cycle-regulated nuclear phosphoprotein. Proc Natl Acad Sci U S A. 1997;94:7138-43 pubmed

product information

Catalog Number :

12341

Product Name :

pCL-MFG-BRCA1

article :

doi	10.1073/pnas.94.14.7138
id	775
pubmed_id	9207057

bacterial resistance :

Ampicillin

cloning :

backbone

pCLMFG

backbone_mutation

backbone_origin

Verma Lab

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

Full-length BRCA1 cDNA was lifted from this construct as a NcoI (partial digest)-XbaI/blunted fragment and inserted into NcoI (partial digest)-cut and BamHI-cut/blunted pCL-MFG retroviral vector. Addgene's quality control sequence analysis identified a sequence discrepancy relative to Genbank reference sequence NM_007294, resulting in a P871L amino acid substitution in the encoded insert.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	XbaI/BamHI
cloning_site_5	NcoI (partial digest)
promoter
sequencing_primer_3
sequencing_primer_5	LXSN primer
site_3_destroyed	1
site_5_destroyed

entrez_gene

aliases	BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53
gene	BRCA1
id	672

genbank_ids

mutation

name

BRCA1

shRNA_sequence

size

species

9606

Homo sapiens