This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCSC-SP-PW-NepX (aka: pBOB-NEPX)
catalog :
12340
citations: 5
Reference
Norambuena A, Sun X, Wallrabe H, Cao R, Sun N, Pardo E, et al. SOD1 mediates lysosome-to-mitochondria communication and its dysregulation by amyloid-β oligomers. Neurobiol Dis. 2022;169:105737 pubmed publisher
Rudenko L, Wallrabe H, Periasamy A, Siller K, Švindrych Z, Seward M, et al. Intraneuronal Tau Misfolding Induced by Extracellular Amyloid-β Oligomers. J Alzheimers Dis. 2019;71:1125-1138 pubmed publisher
Norambuena A, Wallrabe H, McMahon L, Silva A, Swanson E, Khan S, et al. mTOR and neuronal cell cycle reentry: How impaired brain insulin signaling promotes Alzheimer's disease. Alzheimers Dement. 2017;13:152-167 pubmed publisher
Auer Grumbach M, Toegel S, Schabhüttl M, Weinmann D, Chiari C, Bennett D, et al. Rare Variants in MME, Encoding Metalloprotease Neprilysin, Are Linked to Late-Onset Autosomal-Dominant Axonal Polyneuropathies. Am J Hum Genet. 2016;99:607-623 pubmed publisher
Marr R, Guan H, Rockenstein E, Kindy M, Gage F, Verma I, et al. Neprilysin regulates amyloid Beta peptide levels. J Mol Neurosci. 2004;22:5-11 pubmed
product information
Catalog Number :
12340
Product Name :
pCSC-SP-PW-NepX (aka: pBOB-NEPX)
article :
doi10.1385/JMN:22:1-2:5
id856
pubmed_id14742905
bacterial resistance :
Ampicillin
cloning :
backbonepBOB
backbone_mutation
backbone_origin
backbone_size8500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
The sequence and map shown are for the wild-type Nep plasmid. The NepX plasmid contains an inactivating E585V mutation in the Nep gene.
origin :
37
pi :
alt_names
neprilysin
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5LNCX primer
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCALLA, CD10, CMT2T, NEP, SCA43, SFE
geneMME
id4311
genbank_ids
mutationE585V (inactive mutant)
nameNEP-X
shRNA_sequence
size2250
species
9606
Homo sapiens
tags
resistance markers :
548
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA