This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti4 Blast RFP-PNRC1 W300A
catalog :
123298
citations: 1
Reference
Gaviraghi M, Vivori C, Pareja Sanchez Y, Invernizzi F, Cattaneo A, Santoliquido B, et al. Tumor suppressor PNRC1 blocks rRNA maturation by recruiting the decapping complex to the nucleolus. EMBO J. 2018;37: pubmed publisher
product information
Catalog Number :
123298
Product Name :
pLenti4 Blast RFP-PNRC1 W300A
article :
doi10.15252/embj.201899179
id28197157
pubmed_id30373810
bacterial resistance :
Ampicillin
cloning :
backbonepLenti4
backbone_mutationZeocin resistance replaced with Blasticidin resistance
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Two TetO operators exist between CMV and RFP which may impact expression in the presence of TetR. The expression is unaffected in a standard transfection.
growth strain :
Mammalian expression of PNRC1-W300A mutant N-RFP
origin :
37
pi :
alt_names
cloning
entrez_gene
aliasesB4-2, PNAS-145, PROL2, PRR2
genePNRC1
id10957
genbank_ids
mutationW300A
namePNRC1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagturboRFP
resistance markers :
2794
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA