This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti PGK Puro GFP-LC3B G120A
catalog :
123242
citations: 1
Reference
Agrotis A, Pengo N, Burden J, Ketteler R. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy. 2019;15:976-997 pubmed publisher
product information
Catalog Number :
123242
Product Name :
pLenti PGK Puro GFP-LC3B G120A
article :
doi10.1080/15548627.2019.1569925
id28197412
pubmed_id30661429
bacterial resistance :
Ampicillin
cloning :
backbonepLenti PGK GFP Puro (w509-5)
backbone_mutationRemoval of EGFP, insertion of EGFP-LC3B G120A between BamHI and SalI sites.
backbone_origin
backbone_size7849
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
Expression vector with PGK promoter for low expression of EGFP-LC3B G120A. For lentivirus production and stable transduction in mammalian cells.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3SalI
cloning_site_5BamHI
promoterPGK
sequencing_primer_3WPRE-R
sequencing_primer_5EGFP C F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
geneMAP1LC3B
id81631
genbank_ids
NM_022818.4
mutationGlycine 120 to Alanine
nameMicrotubule-associated proteins 1A/1B light chain 3B
shRNA_sequence
size378
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagEGFP
resistance markers :
2021
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA