pDEST-CMV mCherry-GFP-LC3B G120A | Addgene 123235 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pDEST-CMV mCherry-GFP-LC3B G120A

catalog :

123235

citations: 3

Reference
Hu P, Li H, Sun W, Wang H, Yu X, Qing Y, et al. Cholesterol-associated lysosomal disorder triggers cell death of hematological malignancy: Dynamic analysis on cytotoxic effects of LW-218. Acta Pharm Sin B. 2021;11:3178-3192 pubmed publisher
Hu P, Wang J, Qing Y, Li H, Sun W, Yu X, et al. FV-429 induces autophagy blockage and lysosome-dependent cell death of T-cell malignancies via lysosomal dysregulation. Cell Death Dis. 2021;12:80 pubmed publisher
Agrotis A, Pengo N, Burden J, Ketteler R. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy. 2019;15:976-997 pubmed publisher

product information

Catalog Number :

123235

Product Name :

pDEST-CMV mCherry-GFP-LC3B G120A

article :

doi	10.1080/15548627.2019.1569925
id	28197412
pubmed_id	30661429

bacterial resistance :

Ampicillin

cloning :

backbone

pDEST-CMV-N-Tandem-mCherry-EGFP

backbone_mutation

backbone_origin

backbone_size

7000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Expresses mCherry-EGFP-LC3B G120A in mammalian cells. Negative control for tandem reporter. High expression driven by CMV promoter.

origin :

pi :

alt_names

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3	BGH Reverse
sequencing_primer_5	EGFP C F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
gene	MAP1LC3B
id	81631

genbank_ids

NM_022818.4

mutation

Glycine 120 to Alanine

name

Microtubule-associated proteins 1A/1B light chain 3B

shRNA_sequence

size

378

species

9606

Homo sapiens