This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDEST-CMV mCherry-GFP-LC3B WT
catalog :
123230
citations: 9
Reference
Ray N, Park S, Jung H, Kim J, Korcsmáros T, Moon Y. Stress-responsive Gdf15 counteracts renointestinal toxicity via autophagic and microbiota reprogramming. Commun Biol. 2023;6:602 pubmed publisher
Li H, Wang P, Zhang C, Zuo Y, Zhou Y, Han R. Defective BVES-mediated feedback control of cAMP in muscular dystrophy. Nat Commun. 2023;14:1785 pubmed publisher
Liang J, Djurkovic M, Shtanko O, Harty R. Chaperone-assisted selective autophagy targets filovirus VP40 as a client and restricts egress of virus particles. Proc Natl Acad Sci U S A. 2023;120:e2210690120 pubmed publisher
Jain V, Harper S, Versace A, Fingerman D, Brown G, Bhardwaj M, et al. Targeting UGCG overcomes resistance to lysosomal autophagy inhibition. Cancer Discov. 2022;: pubmed publisher
Su W, YU X, Zhou C. SARS-CoV-2 ORF3a Induces Incomplete Autophagy via the Unfolded Protein Response. Viruses. 2021;13: pubmed publisher
Aring L, Choi E, Kopera H, Lanigan T, Iwase S, Klionsky D, et al. A neurodegeneration gene, WDR45, links impaired ferritinophagy to iron accumulation. J Neurochem. 2021;: pubmed publisher
Grisan F, Iannucci L, Surdo N, Gerbino A, Zanin S, Di Benedetto G, et al. PKA compartmentalization links cAMP signaling and autophagy. Cell Death Differ. 2021;28:2436-2449 pubmed publisher
Zang H, Wu W, Qi L, Tan W, Nagarkatti P, Nagarkatti M, et al. Autophagy Inhibition Enables Nrf2 to Exaggerate the Progression of Diabetic Cardiomyopathy in Mice. Diabetes. 2020;69:2720-2734 pubmed publisher
Agrotis A, Pengo N, Burden J, Ketteler R. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy. 2019;15:976-997 pubmed publisher
product information
Catalog Number :
123230
Product Name :
pDEST-CMV mCherry-GFP-LC3B WT
article :
doi10.1080/15548627.2019.1569925
id28197412
pubmed_id30661429
bacterial resistance :
Ampicillin
cloning :
backbonepDEST-CMV-N-Tandem-mCherry-EGFP
backbone_mutation
backbone_origin
backbone_size7000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses mCherry-EGFP-LC3B wild-type in mammalian cells. Fluorescent tandem reporter for autophagosomes. High expression driven by CMV promoter.
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3BGH Reverse
sequencing_primer_5EGFP C F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
geneMAP1LC3B
id81631
genbank_ids
NM_022818.4
mutation
nameMicrotubule-associated proteins 1A/1B light chain 3B
shRNA_sequence
size378
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmCherry
locationN terminal on backbone
tagEGFP
resistance markers :
2021
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA