This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pDEST-CMV 3xFLAG-LC3B-GFP
catalog :
123107
citations: 2
Reference
Tulpule A, Guan J, Neel D, Allegakoen H, Lin Y, Brown D, et al. Kinase-mediated RAS signaling via membraneless cytoplasmic protein granules. Cell. 2021;184:2649-2664.e18 pubmed publisher
Agrotis A, Pengo N, Burden J, Ketteler R. Redundancy of human ATG4 protease isoforms in autophagy and LC3/GABARAP processing revealed in cells. Autophagy. 2019;15:976-997 pubmed publisher
product information
Catalog Number :
123107
Product Name :
pDEST-CMV 3xFLAG-LC3B-GFP
article :
doi10.1080/15548627.2019.1569925
id28197412
pubmed_id30661429
bacterial resistance :
Ampicillin
cloning :
backbonepDEST-CMV-3xFLAG-gateway-EGFP
backbone_mutation
backbone_origin
backbone_size6111
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses 3xFLAG-LC3B-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3EGFP Nrev
sequencing_primer_5CMVF_pCDNA3
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a
geneMAP1LC3B
id81631
genbank_ids
NM_022818.4
mutation
nameMicrotubule-associated proteins 1A/1B light chain 3B
shRNA_sequence
size378
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag3xFLAG
locationC terminal on backbone
tagEGFP
resistance markers :
2021
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA