This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
eGFP STAT1 S727E
catalog :
12305
citations: 2
Reference
Nitulescu I, Meyer S, Wen Q, Crispino J, Lemieux M, Levine R, et al. Mediator Kinase Phosphorylation of STAT1 S727 Promotes Growth of Neoplasms With JAK-STAT Activation. EBioMedicine. 2017;26:112-125 pubmed publisher
Timofeeva O, Plisov S, Evseev A, Peng S, Jose Kampfner M, Lovvorn H, et al. Serine-phosphorylated STAT1 is a prosurvival factor in Wilms' tumor pathogenesis. Oncogene. 2006;25:7555-64 pubmed
product information
Catalog Number :
12305
Product Name :
eGFP STAT1 S727E
article :
doi10.1038/sj.onc.1209742
id848
pubmed_id16799645
bacterial resistance :
Kanamycin
cloning :
backbonepEGFP-C1
backbone_mutation
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
The STAT1 full-size coding region was amplified using total cDNA from SK-NEP-1 cells and cloned into pEGFP-C1. S727E mutation made using QuikChange mutagenesis kit. STAT1 also contains N372D mutation compared to all available reference sequences.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BglII
promoter
sequencing_primer_3
sequencing_primer_5EGFP-C (5'-CATGGTCCTGCTGGAGTTCGTG-3')
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCANDF7, IMD31A, IMD31B, IMD31C, ISGF-3, STAT91
geneSTAT1
id6772
genbank_ids
NM_007315
mutationS727E
nameSTAT1 S727E
shRNA_sequence
size2300
species
9606
Homo sapiens
tags
locationN terminal on backbone
tageGFP
resistance markers :
389
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA