This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV hSyn syph-GFP-myc-BoNT/B(1-146)-iLID
catalog :
122981
citations: 2
Reference
Choi J, Kim J, Kim J. Capturing activated neurons and synapses. Neurosci Res. 2019;: pubmed publisher
Liu Q, Sinnen B, Boxer E, Schneider M, Grybko M, Buchta W, et al. A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission. Neuron. 2019;101:863-875.e6 pubmed publisher
product information
Catalog Number :
122981
Product Name :
pAAV hSyn syph-GFP-myc-BoNT/B(1-146)-iLID
article :
doi10.1016/j.neuron.2019.01.002
id28197448
pubmed_id30704911
bacterial resistance :
Ampicillin
cloning :
backbonepAAV hSyn
backbone_mutation
backbone_origin
backbone_size4500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
growth strain :
AAV plasmid with human synapsin promoter driving synaptophysin fused to GFP, iLID(V416I) and BoNT/B amino acids 1-146. Co-express with SSPB-BoNT(147-441, Y365A) for vPA-BoNT
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRV
cloning_site_5NheI
promoterhuman synapsin
sequencing_primer_3ccacatagcgtaaaaggagcaac
sequencing_primer_5actcagcgctgcctcagt
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesSyp1
geneSyp
id24804
genbank_ids
mutation
nameSyph-GFP-myc-BoNT/B(1-146)-iLID
shRNA_sequence
size2700
species
10116
Rattus norvegicus
100
Synthetic
tags
location
tagGFP
plasmid copy :
iLID was obtained from Dr. Brian Kuhlman, University of North Carolina Chapel Hill BoNT/B was a gift from Dr. Thomas Binz, Hannover Medical School
resistance markers :
1770
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA