This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pIRESneo-EGFP-alpha Tubulin
catalog :
12298
citations: 16
Reference
Ruba A, Tingey M, Luo W, Yu J, Evangelou A, Higgins R, et al. The ciliary lumen accommodates passive diffusion and vesicle-assisted trafficking in cytoplasm-ciliary transport. Mol Biol Cell. 2023;34:ar59 pubmed publisher
Mendon N, Ganie R, Kesarwani S, Dileep D, Sasi S, Lama P, et al. Nanobody derived using a peptide epitope from the spike protein receptor-binding motif inhibits entry of SARS-CoV-2 variants. J Biol Chem. 2022;299:102732 pubmed publisher
Oliva M, Tosat Bitrián C, Barrado Gil L, Bonato F, Galindo I, Garaigorta U, et al. Effect of Clinically Used Microtubule Targeting Drugs on Viral Infection and Transport Function. Int J Mol Sci. 2022;23: pubmed publisher
Zhang X, Vigers M, McCarty J, Rauch J, Fredrickson G, Wilson M, et al. The proline-rich domain promotes Tau liquid-liquid phase separation in cells. J Cell Biol. 2020;219: pubmed publisher
Ma G, He L, Liu S, Xie J, Huang Z, Jing J, et al. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun. 2020;11:1039 pubmed publisher
Subramanian A, Kanzaki L, Galloway J, Schilling T. Mechanical force regulates tendon extracellular matrix organization and tenocyte morphogenesis through TGFbeta signaling. elife. 2018;7: pubmed publisher
Dong Z, Wu S, Zhu C, Wang X, Li Y, Chen X, et al. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9-mediated kif15 mutations accelerate axonal outgrowth during neuronal development and regeneration in zebrafish. Traffic. 2019;20:71-81 pubmed publisher
Zhong X, Lee H, Kim S, Park S, Kim W, Cha Y, et al. Myc-nick promotes efferocytosis through M2 macrophage polarization during resolution of inflammation. FASEB J. 2018;32:5312-5325 pubmed publisher
Weatherly L, Nelson A, Shim J, Riitano A, Gerson E, Hart A, et al. Antimicrobial agent triclosan disrupts mitochondrial structure, revealed by super-resolution microscopy, and inhibits mast cell signaling via calcium modulation. Toxicol Appl Pharmacol. 2018;349:39-54 pubmed publisher
Luo W, Ruba A, Takao D, Zweifel L, Lim R, Verhey K, et al. Axonemal Lumen Dominates Cytosolic Protein Diffusion inside the Primary Cilium. Sci Rep. 2017;7:15793 pubmed publisher
Tocco V, Li Y, Christopher K, Matthews J, Aggarwal V, Paschall L, et al. The nucleus is irreversibly shaped by motion of cell boundaries in cancer and non-cancer cells. J Cell Physiol. 2018;233:1446-1454 pubmed publisher
Gavara N, Chadwick R. Relationship between cell stiffness and stress fiber amount, assessed by simultaneous atomic force microscopy and live-cell fluorescence imaging. Biomech Model Mechanobiol. 2016;15:511-23 pubmed publisher
Wang J, Li J, Santana Santos L, Shuda M, Sobol R, Van Houten B, et al. A novel strategy for targeted killing of tumor cells: Induction of multipolar acentrosomal mitotic spindles with a quinazolinone derivative mdivi-1. Mol Oncol. 2015;9:488-502 pubmed publisher
Svensson K, Christianson H, Wittrup A, Bourseau Guilmain E, Lindqvist E, Svensson L, et al. Exosome uptake depends on ERK1/2-heat shock protein 27 signaling and lipid Raft-mediated endocytosis negatively regulated by caveolin-1. J Biol Chem. 2013;288:17713-24 pubmed publisher
Celik E, Abdulreda M, Maiguel D, Li J, Moy V. Rearrangement of microtubule network under biochemical and mechanical stimulations. Methods. 2013;60:195-201 pubmed publisher
Rusan N, Fagerstrom C, Yvon A, Wadsworth P. Cell cycle-dependent changes in microtubule dynamics in living cells expressing green fluorescent protein-alpha tubulin. Mol Biol Cell. 2001;12:971-80 pubmed
product information
Catalog Number :
12298
Product Name :
pIRESneo-EGFP-alpha Tubulin
article :
doi10.1091/mbc.12.4.971
id846
pubmed_id11294900
bacterial resistance :
Ampicillin
cloning :
backbonepIRESneo2
backbone_mutation
backbone_originClontech
backbone_size5300
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
EGFP-Tubulin came from pEGFP-alpha Tubulin from Clontech
origin :
37
pi :
alt_names
EGFP-Tubulin
k-alpha-1
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NheI
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesK-ALPHA-1
geneTUBA1B
id10376
genbank_ids
mutation
nameEGFP-alpha Tubulin
shRNA_sequence
size2100
species
9606
Homo sapiens
tags
locationN terminal on insert
tagEGFP
resistance markers :
557
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA