This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pWZL Blast H-Ras G12V Y40C
catalog :
12279
citations: 1
Reference
Rangarajan A, Hong S, Gifford A, Weinberg R. Species- and cell type-specific requirements for cellular transformation. Cancer Cell. 2004;6:171-83 pubmed
product information
Catalog Number :
12279
Product Name :
pWZL Blast H-Ras G12V Y40C
article :
doi10.1016/j.ccr.2004.07.009
id833
pubmed_id15324700
bacterial resistance :
Ampicillin
cloning :
backbonepWZL Blast
backbone_mutation
backbone_origin
backbone_size5100
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Retroviral
origin :
37
pi :
alt_names
HRas
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5EcoRI
promoter
sequencing_primer_3
sequencing_primer_5pBABE 5'
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
geneHRAS
id3265
genbank_ids
mutationContains G12V activating mutation. The Y40C mutation allows binding and activation of PI3K, but not of Raf or Ral-GEF.
nameH-Ras G12V Y40C
shRNA_sequence
size
species
9606
Homo sapiens
tags
plasmid copy :
See Rodriguez-Viciana, P., et al. (1997) Cell 89, pp. 457
resistance markers :
568
tags :
High Copy
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA