pWZL Blast H-Ras G12V E37G | Addgene 12277 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pWZL Blast H-Ras G12V E37G

catalog :

12277

citations: 2

Reference
Avila A, Illing A, Becker F, Maerz L, Morita Y, Philipp M, et al. Xpg limits the expansion of haematopoietic stem and progenitor cells after ionising radiation. Nucleic Acids Res. 2016;44:6252-61 pubmed publisher
Rangarajan A, Hong S, Gifford A, Weinberg R. Species- and cell type-specific requirements for cellular transformation. Cancer Cell. 2004;6:171-83 pubmed

product information

Catalog Number :

12277

Product Name :

pWZL Blast H-Ras G12V E37G

article :

doi	10.1016/j.ccr.2004.07.009
id	833
pubmed_id	15324700

bacterial resistance :

Ampicillin

cloning :

backbone

pWZL Blast

backbone_mutation

backbone_origin

backbone_size

5100

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

origin :

pi :

alt_names

HRas

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	EcoRI
promoter
sequencing_primer_3
sequencing_primer_5	pBABE 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
gene	HRAS
id	3265

genbank_ids

mutation

Contains G12V activating mutation. The E37G mutation allows binding and activation of Ral-GEFs, but not of Raf or PI3K.

name

H-Ras G12V E37G

shRNA_sequence

size

species

9606

Homo sapiens