pBabe puro H-Ras G12V Y40C | Addgene 12276 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBabe puro H-Ras G12V Y40C

catalog :

12276

citations: 5

Reference
Getachew A, Abbas N, You K, Yang Z, Hussain M, Huang X, et al. SAA1/TLR2 axis directs chemotactic migration of hepatic stellate cells responding to injury. iScience. 2021;24:102483 pubmed publisher
Becker L, Takwi A, Lu Z, Li Y. The role of miR-200a in mammalian epithelial cell transformation. Carcinogenesis. 2015;36:2-12 pubmed publisher
Cipriano R, Miskimen K, Bryson B, Foy C, Bartel C, Jackson M. FAM83B-mediated activation of PI3K/AKT and MAPK signaling cooperates to promote epithelial cell transformation and resistance to targeted therapies. Oncotarget. 2013;4:729-38 pubmed
Liu Y, Wang D, Chen S, Zhao L, Sun F. Oncogene Ras/phosphatidylinositol 3-kinase signaling targets histone H3 acetylation at lysine 56. J Biol Chem. 2012;287:41469-80 pubmed publisher
Rangarajan A, Hong S, Gifford A, Weinberg R. Species- and cell type-specific requirements for cellular transformation. Cancer Cell. 2004;6:171-83 pubmed

product information

Catalog Number :

12276

Product Name :

pBabe puro H-Ras G12V Y40C

article :

doi	10.1016/j.ccr.2004.07.009
id	833
pubmed_id	15324700

bacterial resistance :

Ampicillin

cloning :

backbone

pBABE-puro

backbone_mutation

backbone_origin

backbone_size

5150

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

A BamHI-SalI fragment from the pWZL Blast ras mutants were individually cloned into the pBABE puro vector directionally.

origin :

pi :

alt_names

HRas

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	BamHI
promoter
sequencing_primer_3	pBABE 3'
sequencing_primer_5	pBABE 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
gene	HRAS
id	3265

genbank_ids

mutation

Contains G12V activating mutation. The Y40C mutation allows binding and activation of PI3K, but not of Raf or Ral-GEF.

name

H-Ras G12V Y40C

shRNA_sequence

size

species

9606

Homo sapiens