pBabe puro H-Ras G12V T35S | Addgene 12274 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBabe puro H-Ras G12V T35S

catalog :

12274

citations: 8

Reference
Gupta R, Forloni M, Bisserier M, Dogra S, Yang Q, Wajapeyee N. Interferon alpha-inducible protein 6 regulates NRASQ61K-induced melanomagenesis and growth. elife. 2016;5: pubmed publisher
Kim R, Kang M, Kim T, Yang P, Bae S, Williams D, et al. Regulation of p53 during senescence in normal human keratinocytes. Aging Cell. 2015;14:838-46 pubmed publisher
Becker L, Takwi A, Lu Z, Li Y. The role of miR-200a in mammalian epithelial cell transformation. Carcinogenesis. 2015;36:2-12 pubmed publisher
Tamashiro P, Furuya H, Shimizu Y, Kawamori T. Sphingosine kinase 1 mediates head & neck squamous cell carcinoma invasion through sphingosine 1-phosphate receptor 1. Cancer Cell Int. 2014;14:76 pubmed publisher
Forloni M, Dogra S, Dong Y, Conte D, Ou J, Zhu L, et al. miR-146a promotes the initiation and progression of melanoma by activating Notch signaling. elife. 2014;3:e01460 pubmed publisher
Cipriano R, Miskimen K, Bryson B, Foy C, Bartel C, Jackson M. FAM83B-mediated activation of PI3K/AKT and MAPK signaling cooperates to promote epithelial cell transformation and resistance to targeted therapies. Oncotarget. 2013;4:729-38 pubmed
Liu Y, Wang D, Chen S, Zhao L, Sun F. Oncogene Ras/phosphatidylinositol 3-kinase signaling targets histone H3 acetylation at lysine 56. J Biol Chem. 2012;287:41469-80 pubmed publisher
Rangarajan A, Hong S, Gifford A, Weinberg R. Species- and cell type-specific requirements for cellular transformation. Cancer Cell. 2004;6:171-83 pubmed

product information

Catalog Number :

12274

Product Name :

pBabe puro H-Ras G12V T35S

article :

doi	10.1016/j.ccr.2004.07.009
id	833
pubmed_id	15324700

bacterial resistance :

Ampicillin

cloning :

backbone

pBABE-puro

backbone_mutation

backbone_origin

backbone_size

5150

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Retroviral

growth notes :

A BamHI-SalI fragment from the pWZL Blast ras mutants were individually cloned into the pBABE puro vector directionally.

origin :

pi :

alt_names

HRas

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	BamHI
promoter
sequencing_primer_3	pBABE 3'
sequencing_primer_5	pBABE 5'
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
gene	HRAS
id	3265

genbank_ids

mutation

Contains G12V activating mutation. The T35S mutation allows binding and activation of Raf, but not of PI3K or Ral-GEF.

name

H-Ras G12V T35S

shRNA_sequence

size

species

9606

Homo sapiens