This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
M31: pHR-SFFVp- TDP-43C::mCherry::SspB
catalog :
122669
citations: 1
Reference
Bracha D, Walls M, Wei M, Zhu L, Kurian M, Avalos J, et al. Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds. Cell. 2018;175:1467-1480.e13 pubmed publisher
product information
Catalog Number :
122669
Product Name :
M31: pHR-SFFVp- TDP-43C::mCherry::SspB
article :
doi10.1016/j.cell.2018.10.048
id28197291
pubmed_id30500534
bacterial resistance :
Ampicillin
cloning :
backbonepHR
backbone_mutation
backbone_origin
backbone_size9000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Vector contains SspB, a bacterial protein which binds exposed SsrA tag (from iLID) at high affinity.
growth strain :
C terminal domain of TDP43 (218-414) tagged with SspB and mCherry.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterSFFV
sequencing_primer_3n/a
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALS10, TDP-43
geneTARDBP
id23435
genbank_ids
mutationContains only amino acids 218-414
nameTDP43
shRNA_sequence
size591
species
9606
Homo sapiens
tags
locationC terminal on insert
tagmCherry-SspB
resistance markers :
3695
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA