pHR-CMV-nlsCRE | Addgene 12265 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pHR-CMV-nlsCRE

catalog :

12265

citations: 6

Reference
Iaffaldano B, Marino M, Reiser J. CRISPR library screening to develop HEK293-derived cell lines with improved lentiviral vector titers. Front Genome Ed. 2023;5:1218328 pubmed publisher
Merle N, Elmsh xe4 user S, Strassheimer F, Wanzel M, K xf6 nig A, Funk J, et al. Monitoring autochthonous lung tumors induced by somatic CRISPR gene editing in mice using a secreted luciferase. Mol Cancer. 2022;21:191 pubmed publisher
Johnson N, Alvarado A, Moffatt T, Edavettal J, Swaminathan T, Braun S. HIV-based lentiviral vectors: origin and sequence differences. Mol Ther Methods Clin Dev. 2021;21:451-465 pubmed publisher
De la Cuesta F, Passalacqua I, Rodor J, Bhushan R, Denby L, Baker A. Extracellular vesicle cross-talk between pulmonary artery smooth muscle cells and endothelium during excessive TGF-β signalling: implications for PAH vascular remodelling. Cell Commun Signal. 2019;17:143 pubmed publisher
Eritja N, Felip I, Dosil M, Vigezzi L, Mirantes C, Yeramian A, et al. A Smad3-PTEN regulatory loop controls proliferation and apoptotic responses to TGF-? in mouse endometrium. Cell Death Differ. 2017;24:1443-1458 pubmed publisher
Cudré Mauroux C, Occhiodoro T, Konig S, Salmon P, Bernheim L, Trono D. Lentivector-mediated transfer of Bmi-1 and telomerase in muscle satellite cells yields a duchenne myoblast cell line with long-term genotypic and phenotypic stability. Hum Gene Ther. 2003;14:1525-33 pubmed

product information

Catalog Number :

12265

Product Name :

pHR-CMV-nlsCRE

article :

doi	10.1089/104303403322495034
id	835
pubmed_id	14577914

bacterial resistance :

Ampicillin

cloning :

backbone

pHR-CRE

backbone_mutation

backbone_origin

backbone_size

9795

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

Cre/Lox

growth notes :

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

growth temp :

Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	CMV forward
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

Cre

shRNA_sequence

size

species