This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pWPXL
catalog :
12257
citations: 65
Reference
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Zhang S, Wang Y, Xu J, Kim B, Deng W, Guo F. HIFα Regulates Developmental Myelination Independent of Autocrine Wnt Signaling. J Neurosci. 2021;41:251-268 pubmed publisher
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Escoll M, Lastra D, Robledinos Antón N, Wandosell F, Anton I, Cuadrado A. WIP Modulates Oxidative Stress through NRF2/KEAP1 in Glioblastoma Cells. Antioxidants (Basel). 2020;9: pubmed publisher
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Velasco C, Llobet A. Synapse elimination activates a coordinated homeostatic presynaptic response in an autaptic circuit. Commun Biol. 2020;3:260 pubmed publisher
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Aldaz P, Otaegi Ugartemendia M, Sáenz Antoñanzas A, Garcia Puga M, Moreno Valladares M, Flores J, et al. SOX9 promotes tumor progression through the axis BMI1-p21CIP. Sci Rep. 2020;10:357 pubmed publisher
Escoll M, Lastra D, Pajares M, Robledinos Antón N, Rojo A, Fernández Ginés R, et al. Transcription factor NRF2 uses the Hippo pathway effector TAZ to induce tumorigenesis in glioblastomas. Redox Biol. 2020;30:101425 pubmed publisher
Martinez San Segundo P, Terni B, Burgueno J, Monroy X, Dordal A, Merlos M, et al. Outside-in regulation of the readily releasable pool of synaptic vesicles by α2δ-1. FASEB J. 2020;34:1362-1377 pubmed publisher
Kriesel F, Stegmann L, Ciesek S, Kaderali L, Baldauf H. Efficient inactivation of pseudotyped HIV-based lentiviral vectors and infectious HIV. J Virol Methods. 2020;276:113768 pubmed publisher
Legewie L, Loschwitz J, Steffens N, Prescher M, Wang X, Smits S, et al. Biochemical and structural characterization of murine GBP7, a guanylate binding protein with an elongated C-terminal tail. Biochem J. 2019;476:3161-3182 pubmed publisher
Farmer L, Rollason R, Whitcomb D, Ni L, Goodliff A, Lay A, et al. TRPC6 Binds to and Activates Calpain, Independent of Its Channel Activity, and Regulates Podocyte Cytoskeleton, Cell Adhesion, and Motility. J Am Soc Nephrol. 2019;30:1910-1924 pubmed publisher
Yang W, Redpath R, Zhang C, Ning N. Long non-coding RNA H19 promotes the migration and invasion of colon cancer cells via MAPK signaling pathway. Oncol Lett. 2018;16:3365-3372 pubmed publisher
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Wang C, Zeng N, Liu S, Miao Q, Zhou L, Ge X, et al. Interaction of porcine reproductive and respiratory syndrome virus proteins with SUMO-conjugating enzyme reveals the SUMOylation of nucleocapsid protein. PLoS ONE. 2017;12:e0189191 pubmed publisher
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Zhang X, Jiang S, Mitok K, Li L, Attie A, Martin T. BAIAP3, a C2 domain-containing Munc13 protein, controls the fate of dense-core vesicles in neuroendocrine cells. J Cell Biol. 2017;216:2151-2166 pubmed publisher
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product information
Catalog Number :
12257
Product Name :
pWPXL
article :
doi
id829
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepWPXL
backbone_mutation
backbone_origin
backbone_size10510
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
Cre/Lox
growth notes :
pWPT or pWPXL can be used for constitutive transgene expression. pWPXL contains the EF-1alpha promoter + intron that gives you high expression, as RNA loves to be spliced (it goes more efficiently out of the nucleus). pWPT contains only the EF-1alpha promoter The loxP site in the 3'LTR is duplicated to the 5'LTR during reverse transcription in the target cells. This allows for removal (if necessary) of an integrated provirus by Cre. Unique restriction sites at key positions will allow you to change promoter and transgene. PmeI is a popular site for cloning. You can also use PacI and SwaI. Please note that ClaI in this vector is blocked by Dam methylation. This plasmid needs to be grown in a Dam- bacteria strain if you wish to use ClaI for cloning. Packaging plasmids for Trono lab lentiviral vectors are also available at Addgene http://www.addgene.org/rnaitools Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussions on cloning strategies and protocols.
growth strain :
2nd generation lentiviral transfer plasmid
growth temp :
Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3MCS - see map
cloning_site_5MCS - see map
promoter
sequencing_primer_3
sequencing_primer_5See map
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
name
shRNA_sequence
size
species
tags
location
tagEGFP
resistance markers :
532
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA