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product summary
company name :
Addgene
product type :
cDNA
product name :
pRSV-Rev
catalog :
12253
citations: 715
Reference
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product information
Catalog Number :
12253
Product Name :
pRSV-Rev
article :
doi
id838
pubmed_id9765382
bacterial resistance :
Ampicillin
cloning :
backbonepRSV-Rev
backbone_mutation
backbone_origin
backbone_size4174
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
Other
Packaging
growth notes :
Rev cDNA expressing plasmid in which the joined second and third exons of HIV-1 rev are under the transcriptional control of RSV U3 promoter. Part of the 3rd generation packaging system. Please note that most of Trono lab lentiviral vectors can only be used with the 2nd generation packaging system. See notes for pMDLg/pRRE. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussions on cloning strategies and protocols.
growth strain :
3rd generation lentiviral packaging plasmid; Contains Rev; also requires pMDLg/pRRE (Addgene#12251) and envelope expressing plasmid (Addgene#12259)
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5pREP fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHIV1gp6
generev
id155908
genbank_ids
mutation
nameRev
shRNA_sequence
size
species
tags
resistance markers :
532
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA