This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCR2.1 Clover-LMNA Donor
catalog :
122508
citations: 5
Reference
Zhu Y, Liu Z, Gui L, Yun W, Mao C, Deng R, et al. Inhibition of CXorf56 promotes PARP inhibitor-induced cytotoxicity in triple-negative breast cancer. NPJ Breast Cancer. 2023;9:34 pubmed publisher
Carusillo A, Haider S, Sch xe4 fer R, Rhiel M, T xfc rk D, Chmielewski K, et al. A novel Cas9 fusion protein promotes targeted genome editing with reduced mutational burden in primary human cells. Nucleic Acids Res. 2023;51:4660-4673 pubmed publisher
Chansel Da Cruz M, Hohl M, Ceppi I, Kermasson L, Maggiorella L, Modesti M, et al. A Disease-Causing Single Amino Acid Deletion in the Coiled-Coil Domain of RAD50 Impairs MRE11 Complex Functions in Yeast and Humans. Cell Rep. 2020;33:108559 pubmed publisher
Attwood K, Salsman J, Chung D, Mathavarajah S, Van Iderstine C, Dellaire G. PML isoform expression and DNA break location relative to PML nuclear bodies impacts the efficiency of homologous recombination. Biochem Cell Biol. 2019;: pubmed publisher
Pinder J, Salsman J, Dellaire G. Nuclear domain 'knock-in' screen for the evaluation and identification of small molecule enhancers of CRISPR-based genome editing. Nucleic Acids Res. 2015;43:9379-92 pubmed publisher
product information
Catalog Number :
122508
Product Name :
pCR2.1 Clover-LMNA Donor
article :
doi10.1093/nar/gkv993
id17228
pubmed_id26429972
bacterial resistance :
Ampicillin
cloning :
backbonepCR2.1 TOPO
backbone_mutation
backbone_originInvitrogen
backbone_size3900
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Other
Homology Repair Template Plasmid (Donor Plasmid)
growth strain :
Homology repair template for in frame (first exon) clover knock-in of human LMNA gene
growth temp :
Kanamycin Resistance as well
origin :
37
pi :
alt_names
cloning
clone_methodTOPO Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13-Fwd
sequencing_primer_5M13-Reverse
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, FPLD2, HGPS, IDC, LDP1, LFP, LGMD1B, LMN1, LMNC, LMNL1, MADA, PRO1
geneLMNA
id4000
genbank_ids
mutation
nameHomology Repair Template for Human LMNA (N-Terminal Clover Tag)
shRNA_sequence
size1837
species
9606
Homo sapiens
32644
Other
tags
location
tagClover
resistance markers :
4395
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA