This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti/TO/EYFP-P2A-IDH1(HA)
catalog :
122482
citations: 1
Reference
Tiburcio P, Xiao B, Berg S, Asper S, Lyne S, Zhang Y, et al. Functional requirement of a wild-type allele for mutant IDH1 to suppress anchorage-independent growth through redox homeostasis. Acta Neuropathol. 2018;135:285-298 pubmed publisher
product information
Catalog Number :
122482
Product Name :
pLenti/TO/EYFP-P2A-IDH1(HA)
article :
doi10.1007/s00401-017-1800-0 [pii]
id28197359
pubmed_id29288440
bacterial resistance :
Ampicillin
cloning :
backbonepLenti/TO/DEST
backbone_mutationThe V5 tag was replaced by a 3xFLAG sequence
backbone_originInvitrogen
backbone_size7725
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth strain :
A lentiviral vector (pLenti6.3/TO/DEST) expresses EYFP, P2A, and IDH1 C-terminally tagged with HA
origin :
37
pi :
alt_names
EYFP-IDH1
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV/TO
sequencing_primer_3CAGAGGTTGATTGTCGAT
sequencing_primer_5GTGTACGGTGGGAGGTCTATATAA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEL-216, HEL-S-26, IDCD, IDH, IDP, IDPC, PICD
geneIDH1
id3417
genbank_ids
mutation
nameisocitrate dehydrogenase 1
shRNA_sequence
size2163
species
9606
Homo sapiens
tags
location
tagP2A
locationC terminal on insert
tagHA
resistance markers :
1881
tags :
Unknown
terms :
Blasticidin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA