HA-IFITM3-F8-TAG-C72A | Addgene 122477 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

HA-IFITM3-F8-TAG-C72A

catalog :

122477

citations: 1

Reference
Spence J, He R, Hoffmann H, Das T, Thinon E, Rice C, et al. IFITM3 directly engages and shuttles incoming virus particles to lysosomes. Nat Chem Biol. 2019;15:259-268 pubmed publisher

product information

Catalog Number :

122477

Product Name :

HA-IFITM3-F8-TAG-C72A

article :

doi	10.1038/s41589-018-0213-2 [pii]
id	28197355
pubmed_id	30643282

bacterial resistance :

Ampicillin

cloning :

backbone

pCMV-HA

backbone_mutation

backbone_origin

clontech

backbone_size

3800

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

expresses human IFITM3-C72A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	SalI
cloning_site_5	EcoRI
promoter
sequencing_primer_3
sequencing_primer_5	GATCCGGTACTAGAGGAACTGAAAAAC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	1-8U, DSPA2b, IP15
gene	IFITM3
id	10410

genbank_ids

mutation

Changed Phenylalanine 8 to amber codon, cysteine 72 to alanine

name

IFITM3

shRNA_sequence

size

species

9606

Homo sapiens