This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFRT/TO/FLAGHA-SCAF8
catalog :
122470
citations: 2
Reference
Gregersen L, Mitter R, Svejstrup J. Elongation factor-specific capture of RNA polymerase II complexes. Cell Rep Methods. 2022;2:100368 pubmed publisher
Gregersen L, Mitter R, Ugalde A, Nojima T, Proudfoot N, Agami R, et al. SCAF4 and SCAF8, mRNA Anti-Terminator Proteins. Cell. 2019;: pubmed publisher
product information
Catalog Number :
122470
Product Name :
pFRT/TO/FLAGHA-SCAF8
article :
doi10.1016/j.cell.2019.04.038
id28197357
pubmed_id31104839
bacterial resistance :
Ampicillin
cloning :
backbonepFRT/TO/FLAGHA
backbone_mutation
backbone_origin
backbone_size5294
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Dox-inducible FLAG/HA (N-terminal tag) SCAF8 expression
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3BGH Reverse
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRBM16
geneSCAF8
id22828
genbank_ids
NM_001286189.1
mutationSynonymous mutations conferring resistance to gRNAs described in Gregersen et al.
nameSCAF8 isoform C CRISPR resistant
shRNA_sequence
size3951
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagFLAGHA
resistance markers :
4348
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA