This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMCRW1
catalog :
122462
citations: 1
Reference
Kourentzi K, Crum M, Patil U, Prebisch A, Chavan D, Vu B, et al. Recombinant expression, characterization, and quantification in human cancer cell lines of the Anaplastic Large-Cell Lymphoma-characteristic NPM-ALK fusion protein. Sci Rep. 2020;10:5078 pubmed publisher
product information
Catalog Number :
122462
Product Name :
pMCRW1
article :
doi10.1038/s41598-020-61936-w
id28197356
pubmed_id32193476
bacterial resistance :
Kanamycin
cloning :
backbonepET28a
backbone_mutation
backbone_origin
backbone_size5289
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Human NPM-ALK fusion protein gene in pET28a bacterial expression plasmid. Production of recombinant human NPM-ALK fusion protein in E. coli Bl21 Rosetta2 cells
origin :
37
pi :
alt_names
NPM-ALK
Anaplastic Large Cell Lymphoma characteristic fusion protein
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoterT7lac promoter
sequencing_primer_3gctagttattgctcagcgg
sequencing_primer_5taatacgactcactatagggg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALK1, CD246, NBLST3
geneALK
id238
aliasesB23, NPM
geneNPM1
id4869
genbank_ids
mutation
nameNPM-ALK fusion protein
shRNA_sequence
size2062
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis Tag
resistance markers :
4387
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA