This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pHR-FUSN-miRFP670-Cry2WT
catalog :
122441
citations: 1
Reference
Shin Y, Chang Y, Lee D, Berry J, Sanders D, Ronceray P, et al. Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Cell. 2018;175:1481-1491.e13 pubmed publisher
product information
Catalog Number :
122441
Product Name :
pHR-FUSN-miRFP670-Cry2WT
article :
doi10.1016/j.cell.2018.10.057
id28197253
pubmed_id30500535
bacterial resistance :
Ampicillin
cloning :
backbonepHR
backbone_mutation
backbone_origin
backbone_size9000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Our Cry2WT contains an additional 29 amino acids at the C-terminus of Cry2PHR the same as our previously deposited plasmid Addgene #101223 (Shin et al., Cell, 2017).
growth strain :
Expresses disordered protein FUS(1-214) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterSFFV
sequencing_primer_3n/a
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS
geneFUS
id2521
genbank_ids
mutationContains only amino acids 1-214
nameFUS
shRNA_sequence
size642
species
9606
Homo sapiens
tags
locationC terminal on insert
tagmiRFP670
locationC terminal on insert
tagCry2WT
plasmid copy :
miRFP670 was from Addgene plasmid #79987 (Shcherbakova et al., Nat. Commun., 2016).
resistance markers :
3695
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA