pLVTH-sip53 | Addgene 12239 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLVTH-sip53

catalog :

12239

citations: 8

Reference
Roy S, Laroche Clary A, Verbeke S, Derieppe M, Italiano A. MDM2 Antagonists Induce a Paradoxical Activation of Erk1/2 through a P53-Dependent Mechanism in Dedifferentiated Liposarcomas: Implications for Combinatorial Strategies. Cancers (Basel). 2020;12: pubmed publisher
Wu Q, Li Z, Mellor P, Zhou Y, Anderson D, Liu Q. The role of PTEN - HCV core interaction in hepatitis C virus replication. Sci Rep. 2017;7:3695 pubmed publisher
Marks D, Thomas R, Chin Y, Shah R, Khoo C, Benezra R. Mad2 Overexpression Uncovers a Critical Role for TRIP13 in Mitotic Exit. Cell Rep. 2017;19:1832-1845 pubmed publisher
Jan A, Jansonius B, Delaidelli A, Somasekharan S, Bhanshali F, Vandal M, et al. eEF2K inhibition blocks Aβ42 neurotoxicity by promoting an NRF2 antioxidant response. Acta Neuropathol. 2017;133:101-119 pubmed publisher
Periz G, Lu J, Zhang T, Kankel M, Jablonski A, Kalb R, et al. Regulation of protein quality control by UBE4B and LSD1 through p53-mediated transcription. PLoS Biol. 2015;13:e1002114 pubmed publisher
Verbeke S, Richard E, Monceau E, Schmidt X, Rousseau B, Velasco V, et al. Humanization of the mouse mammary gland by replacement of the luminal layer with genetically engineered preneoplastic human cells. Breast Cancer Res. 2014;16:504 pubmed publisher
Yu X, Cohen D, Chen C. miR-125b Is an adhesion-regulated microRNA that protects mesenchymal stem cells from anoikis. Stem Cells. 2012;30:956-64 pubmed publisher
Wiznerowicz M, Trono D. Conditional suppression of cellular genes: lentivirus vector-mediated drug-inducible RNA interference. J Virol. 2003;77:8957-61 pubmed

product information

Catalog Number :

12239

Product Name :

pLVTH-sip53

article :

doi	10.1128/JVI.77.16.8957-8951.2003
id	840
pubmed_id	12885912

bacterial resistance :

Ampicillin

cloning :

backbone

pLVTH

backbone_mutation

backbone_origin

backbone_size

11166

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

RNAi

Cre/Lox

growth notes :

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

growth temp :

Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C

origin :

pi :

alt_names

p53

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5	See map
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCC7, BMFS5, LFS1, P53, TRP53
gene	TP53
id	7157

genbank_ids

mutation

name

shRNA against p53

shRNA_sequence

size

species

9606

Homo sapiens