pLOX-CW-CRE | Addgene 12238 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pLOX-CW-CRE

catalog :

12238

citations: 1

Reference
Cudré Mauroux C, Occhiodoro T, Konig S, Salmon P, Bernheim L, Trono D. Lentivector-mediated transfer of Bmi-1 and telomerase in muscle satellite cells yields a duchenne myoblast cell line with long-term genotypic and phenotypic stability. Hum Gene Ther. 2003;14:1525-33 pubmed

product information

Catalog Number :

12238

Product Name :

pLOX-CW-CRE

article :

doi	10.1089/104303403322495034
id	835
pubmed_id	14577914

bacterial resistance :

Ampicillin

cloning :

backbone

pHR

backbone_mutation

backbone_origin

backbone_size

9886

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

Cre/Lox

growth notes :

Please note that the full sequence for this plasmid is approximated and not fully verified. ALTHOUGH THE SEQUENCE OF THIS PLASMID SUGGESTS IT'S FULL SIZE IS 9.9KB, OUR TEST DIGEST SHOWS IT IS ACTUALLY 11.5KB. NONETHELESS, OUR CUSTOMERS HAVE BEEN ABLE TO MAKE FUNCTIONAL VIRAL PARTICLES USING THIS PLASMID. Please visit the Trono lab http://tronolab.epfl.ch for cloning strategies, protocols, publications, and more. See LentiWeb http://www.lentiweb.com for discussion on cloning strategies and protocols.

growth temp :

Use Stbl3 or HB101 to reduce chance of recombination. Grow at 37C

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	SP6 for lox site
sequencing_primer_5	CMV forward
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

CMV promoter is upstream of nls-CRE. LoxP in 3' LTR.

name

CRE

shRNA_sequence

size

species