This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSW149
catalog :
122248
citations: 2
| Reference |
|---|
product information
Catalog Number :
122248
Product Name :
pSW149
article :
| doi | 10.1016/j.ymeth.2019.03.024 |
| id | 28197324 |
| pubmed_id | 30926531 |
bacterial resistance :
Ampicillin
cloning :
| backbone | pTYB2 | |
| backbone_mutation | ||
| backbone_origin | NEB | |
| backbone_size | 7474 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
Both plasmids pSW104 and pSW149 are being published in Liu, X., Schuessler, P., Sahoo, A. and Walker, S.E. 2019. Reconstitution and Analyses of RNA Interactions with Eukaryotic Translation Initiation Factors and Ribosomal Preinitiation Complexes. Methods. S1046-2023: 30395-5
growth strain :
pTYB2 containing TIF4631 gene, expresses yeast eIF4G1 as an intein-chitin-binding domain fusion for purification of untagged full-length protein, with or without a fluorescent label on the C-terminus.
growth temp :
Express protein by freshly transforming in BL21-Codon Plus-DE3-RIL cells from agilent.
origin :
37
pi :
|
resistance markers :
| 4442 |
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments