Ad:2CMV_A2R2-N25Ctr | Addgene 122243 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Ad:2CMV_A2R2-N25Ctr

catalog :

122243

citations: 1

Reference
Seelbinder B, Ghosh S, Schneider S, Scott A, Berman A, Goergen C, et al. Nuclear deformation guides chromatin reorganization in cardiac development and disease. Nat Biomed Eng. 2021;5:1500-1516 pubmed publisher

product information

Catalog Number :

122243

Product Name :

Ad:2CMV_A2R2-N25Ctr

article :

doi	10.1038/s41551-021-00823-9
id	28197167
pubmed_id	34857921

bacterial resistance :

Kanamycin

cloning :

backbone

pShuttle-CMV

backbone_mutation

backbone_origin

backbone_size

7469

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Adenoviral

growth notes :

Dominant negative Kash construct is comprised of the C-terminal end of Nesprin-3, including the transmembrane but excluding the Kash domain, and fused to Nesprin2.5 for visual location and to the signal peptide of TOR1A (NM_000113.2) for membrane integration. This vector is meant as integration defective control (into LINC complex) for the vector Ad:2CMV_A2R2-N25K3 (122242). Please note the L451V substitution in Actinin alpha 2 has no functional consequences. Please visit https://www.biorxiv.org/content/early/2018/10/29/455600 for BioRxiv preprint.

growth strain :

Expresses a dominant negative Kash control, that lacks the actual Kash domain for LINC complex integration, in addition to fluorescently tagged (mRuby2) -Actinin-2 on an adenoviral backbone

origin :

pi :

end of Nesprin-3 w/o KashshRNA_sequencesize120species

10090

Mus musculus