Ad:2CMV_A2R2-N25K3 | Addgene 122242 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Ad:2CMV_A2R2-N25K3

catalog :

122242

citations: 1

Reference
Seelbinder B, Ghosh S, Schneider S, Scott A, Berman A, Goergen C, et al. Nuclear deformation guides chromatin reorganization in cardiac development and disease. Nat Biomed Eng. 2021;5:1500-1516 pubmed publisher

product information

Catalog Number :

122242

Product Name :

Ad:2CMV_A2R2-N25K3

article :

doi	10.1038/s41551-021-00823-9
id	28197167
pubmed_id	34857921

bacterial resistance :

Kanamycin

cloning :

backbone

pShuttle-CMV

backbone_mutation

backbone_origin

backbone_size

7469

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Adenoviral

growth notes :

Dominant negative Kash construct is comprised of the C-terminal end of Nesprin-3, including Kash and transmembrane domain, and fused to Nesprin2.5 for visual location and to the signal peptide of TOR1A (NM_000113.2) for membrane integration - CMV::SP-N25-TmKash3 Please visit https://www.biorxiv.org/content/early/2018/10/29/455600 for BioRxiv preprint.

growth strain :

Expresses a dominant negative Kash construct that disrupts LINC complexes in addition to fluorescently tagged (mRuby2) -Actinin-2 on an adenoviral backbone

origin :

pi :

alt_names

Syne3

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	KASH3, nesprin-3
gene	Syne3
id	212073

genbank_ids

NM_001042699.2

mutation

name

C-terminal end of Nesprin-3

shRNA_sequence

size

207

species

10090

Mus musculus