This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Rescue myc-PCDHGC5 mRNA
catalog :
122229
citations: 1
Reference
Li Y, Xiao H, Chiou T, Jin H, Bonhomme B, Miralles C, et al. Molecular and functional interaction between protocadherin-?C5 and GABAA receptors. J Neurosci. 2012;32:11780-97 pubmed publisher
product information
Catalog Number :
122229
Product Name :
Rescue myc-PCDHGC5 mRNA
article :
doi10.1523/JNEUROSCI.0969-12.2012
id28197001
pubmed_id22915120
bacterial resistance :
Ampicillin
cloning :
backbonepCDNA 3.1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Insert was originally cloned in Angel de Blas's lab.
growth strain :
Rescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5NheI
promoter
sequencing_primer_3
sequencing_primer_5unknown
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
genePcdhgc5
id641525
genbank_ids
mutationSilent mutations are in five consecutive codons (ACATCTGAAGCAGTG to ACGTCCGAGGCTGTTboth encoding TSEAV) in the PCDHGC5 sh1 shRNA target site
namePCDHGC5
shRNA_sequence
size
species
10116
Rattus norvegicus
tags
locationN terminal on insert
tag9E10 cMyc epitope (EQKLISEEDL) was inserted between amino acids 103 and 104 of mature protein (after signal peptide cleavage)
resistance markers :
4182
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA