This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1-myc-BirA*-NRASG12V
catalog :
122209
citations: 1
Reference
Adhikari H, Counter C. Interrogating the protein interactomes of RAS isoforms identifies PIP5K1A as a KRAS-specific vulnerability. Nat Commun. 2018;9:3646 pubmed publisher
product information
Catalog Number :
122209
Product Name :
pcDNA3.1-myc-BirA*-NRASG12V
article :
doi10.1038/s41467-018-05692-6 [pii]
id28197316
pubmed_id30194290
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1 myc BioID
backbone_mutation
backbone_origin
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses myc-BirA* fused to NRASG12V in mammalian cells
origin :
37
pi :
alt_names
NRAS
cloning
clone_methodRestriction Enzyme
cloning_site_3BamH1
cloning_site_5EcoR1
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6
geneNRAS
id4893
genbank_ids
4893
mutationchanged G12 to V
nameNRASG12v
shRNA_sequence
size567
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmyc-BirA*
resistance markers :
95
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA