M43: pHR-SFFVp-iLID::EGFP::FTH1 | Addgene 122149 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

M43: pHR-SFFVp-iLID::EGFP::FTH1

catalog :

122149

citations: 2

Reference
Veling M, Nguyen D, Thadani N, Oster M, Rollins N, Brock K, et al. Natural and Designed Proteins Inspired by Extremotolerant Organisms Can Form Condensates and Attenuate Apoptosis in Human Cells. ACS Synth Biol. 2022;11:1292-1302 pubmed publisher
Bracha D, Walls M, Wei M, Zhu L, Kurian M, Avalos J, et al. Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds. Cell. 2018;175:1467-1480.e13 pubmed publisher

product information

Catalog Number :

122149

Product Name :

M43: pHR-SFFVp-iLID::EGFP::FTH1

article :

doi	10.1016/j.cell.2018.10.048
id	28197291
pubmed_id	30500534

bacterial resistance :

Ampicillin

cloning :

backbone

pHR

backbone_mutation

backbone_origin

backbone_size

9000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Vector contains iLID, an engineered LOV2-SsrA protein for light dependent heterodimerization

growth strain :

Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	SFFV
sequencing_primer_3	n/a
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	FHC, FTH, FTHL6, HFE5, PIG15, PLIF
gene	FTH1
id	2495

genbank_ids

mutation

name

FTH1

shRNA_sequence

size

species

9606

Homo sapiens