M23: pHR-SFFVp-FUSN::mCherry::SspB | Addgene 122148 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

M23: pHR-SFFVp-FUSN::mCherry::SspB

catalog :

122148

citations: 2

Reference
Erdel F, Rademacher A, Vlijm R, Tünnermann J, Frank L, Weinmann R, et al. Mouse Heterochromatin Adopts Digital Compaction States without Showing Hallmarks of HP1-Driven Liquid-Liquid Phase Separation. Mol Cell. 2020;78:236-249.e7 pubmed publisher
Bracha D, Walls M, Wei M, Zhu L, Kurian M, Avalos J, et al. Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds. Cell. 2018;175:1467-1480.e13 pubmed publisher

product information

Catalog Number :

122148

Product Name :

M23: pHR-SFFVp-FUSN::mCherry::SspB

article :

doi	10.1016/j.cell.2018.10.048
id	28197291
pubmed_id	30500534

bacterial resistance :

Ampicillin

cloning :

backbone

pHR

backbone_mutation

backbone_origin

backbone_size

9000

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

Lentiviral

growth notes :

Vector contains SspB, a bacterial protein which binds exposed SsrA tag at high affinity

growth strain :

N terminal domain of FUS (1-214) tagged with SspB and mCherry.

origin :

pi :

alt_names

cloning

clone_method	Ligation Independent Cloning
cloning_site_3
cloning_site_5
promoter	SFFV
sequencing_primer_3	n/a
sequencing_primer_5	n/a
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS
gene	FUS
id	2521

genbank_ids

mutation

Contains only amino acids 1-214

name

FUS

shRNA_sequence

size

species

9606

Homo sapiens