This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1
catalog :
122147
citations: 2
Reference
Wang X, Chen C, Badeti S, Cho J, Naghizadeh A, Wang Z, et al. Deletion of ER-retention motif on SARS-CoV-2 spike protein reduces cell hybrid during cell-cell fusion. Cell Biosci. 2021;11:114 pubmed publisher
Bracha D, Walls M, Wei M, Zhu L, Kurian M, Avalos J, et al. Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds. Cell. 2018;175:1467-1480.e13 pubmed publisher
product information
Catalog Number :
122147
Product Name :
M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1
article :
doi10.1016/j.cell.2018.10.048
id28197291
pubmed_id30500534
bacterial resistance :
Ampicillin
cloning :
backbonepHR
backbone_mutation
backbone_origin
backbone_size9000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
Vector contains iLID, an engineered LOV2-SsrA protein for light dependent heterodimerization
growth strain :
Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.
origin :
37
pi :
alt_names
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterSFFV
sequencing_primer_3n/a
sequencing_primer_5n/a
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesFHC, FTH, FTHL6, HFE5, PIG15, PLIF
geneFTH1
id2495
genbank_ids
mutation
nameFTH1
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagNLS-iLID-GFP
resistance markers :
3695
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA