pCMV6M-Pak1 K299R | Addgene 12210 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCMV6M-Pak1 K299R

catalog :

12210

citations: 9

Reference
Davidson A, Tyler J, Hume P, Singh V, Koronakis V. A kinase-independent function of PAK is crucial for pathogen-mediated actin remodelling. PLoS Pathog. 2021;17:e1009902 pubmed publisher
Ostrowska Podhorodecka Z, Ding I, Lee W, Tanic J, Abbasi S, Arora P, et al. Vimentin tunes cell migration on collagen by controlling β1 integrin activation and clustering. J Cell Sci. 2021;: pubmed publisher
Brown T, Hashimoto H, Finseth L, Wood T, Macklin W. PAK1 positively regulates oligodendrocyte morphology and myelination. J Neurosci. 2021;: pubmed publisher
Van Dijk J, Bompard G, Rabeharivelo G, Cau J, Delsert C, Morin N. PAK1 Regulates MEC-17 Acetyltransferase Activity and Microtubule Acetylation during Proplatelet Extension. Int J Mol Sci. 2020;21: pubmed publisher
Bondar V, Adamski C, Onur T, Tan Q, Wang L, Díaz García J, et al. PAK1 regulates ATXN1 levels providing an opportunity to modify its toxicity in spinocerebellar ataxia type 1. Hum Mol Genet. 2018;27:2863-2873 pubmed publisher
Alvarez JuliÃ¡ A, Frasch A, Fuchsova B. Neuronal filopodium formation induced by the membrane glycoprotein M6a (Gpm6a) is facilitated by coronin-1a, Rac1, and p21-activated kinase 1 (Pak1). J Neurochem. 2016;137:46-61 pubmed publisher
Yeh C, Hsu L. Zebrafish diras1 Promoted Neurite Outgrowth in Neuro-2a Cells and Maintained Trigeminal Ganglion Neurons In Vivo via Rac1-Dependent Pathway. Mol Neurobiol. 2016;53:6594-6607 pubmed
Pham H, Vincenti R, Slice L. COX-2 promoter activation by AT1R-Gq-PAK-p38beta signaling in intestinal epithelial cells. Biochim Biophys Acta. 2008;1779:408-13 pubmed publisher
Sells M, Knaus U, Bagrodia S, Ambrose D, Bokoch G, Chernoff J. Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells. Curr Biol. 1997;7:202-10 pubmed

product information

Catalog Number :

12210

Product Name :

pCMV6M-Pak1 K299R

article :

doi	10.1016/S0960-9822(97)70091-5
id	822
pubmed_id	9395435

bacterial resistance :

Ampicillin

cloning :

backbone

pCMV6

backbone_mutation

backbone_origin

backbone_size

4900

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Please note that the reference sequence from the depositor does not have the K299R mutation. Addgene has sequenced this plasmid and verified this mutation. There is also an additional mutation in this plasmid - L516I. The L516I mutation, or SNP, has been present from the very beginning (~1995). The crystal structure of Pak1 uses this clone, so it is present there too. The depositor does not think it affects function in any way. Pak1 may contain a G401S mutation. Depositor states that this mutation should not affect plasmid function.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	SalI
promoter
sequencing_primer_3	GGAACTTCCAAGGCCAGGA
sequencing_primer_5	CTCGTTTAGTGAACCGTCAG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	IDDMSSD, PAKalpha, alpha-PAK, p65-PAK
gene	PAK1
id	5058

genbank_ids

mutation

Catalytically inactive Pak1: K299R

name

Pak1

shRNA_sequence

size

2000

species

9606

Homo sapiens