pCoofy51 | Addgene 122005 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

pCoofy51

catalog :

122005

citations: 1

Reference
Scholz J, Besir H, Strasser C, Suppmann S. A new method to customize protein expression vectors for fast, efficient and background free parallel cloning. BMC Biotechnol. 2013;13:12 pubmed publisher

product information

Catalog Number :

122005

Product Name :

pCoofy51

article :

doi	10.1186/1472-6750-13-12
id	6402
pubmed_id	23410102

bacterial resistance :

Ampicillin

cloning :

backbone

pFastBac

backbone_mutation

backbone_origin

Thermo

backbone_size

5334

promoter

sequencing_primer_3

pFastBac rev:5'TGTTTCAGGTTCAGGGGGAGGTGT 3'

sequencing_primer_5

pFastBac fd: 5' ATTAAAATGATAACCATCTCG 3'

vector_types

Insect Expression

growth notes :

ccdB Survival cells are NOT suitable for the unmodified plasmid containing the ccdB gene. The plasmid is provided in the recommended DB3.1 strain. Please see supplemental file for LP1 and LP2 primers to use for SLIC cloning. A detailed protocol for using pCoofy plasmids is available at https://p4eu.org/wiki . Use one of the following linearization primer pairs for SLIC cloning. Choose one LP1 forward vector primer and one LP2 reverse vector primer: LP1 forward vector primer: SLIC Primer (3C site) 5' GGGCCCCTGGAACAGAACTTCCAG 3' LP2 reverse vector primer: SLIC Primer 5' CGCCATTAACCTGATGTTCTGGGG 3' Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected.

growth strain :

Baculovirus vector for parallel SLIC cloning containing N-terminal TwinStrep with PreScission Cleavage site

inserts :

DB3.1

origin :

pi :

alt_names
cloning
entrez_gene
genbank_ids
mutation
name
shRNA_sequence
size
species
tags

resistance markers :

1471

tags :

High Copy