This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pNIA-CEN-mVenus-FLAG-LANS1-Set2
catalog :
122003
citations: 1
Reference
Lerner A, Hepperla A, Keele G, Meriesh H, Yumerefendi H, Restrepo D, et al. An optogenetic switch for the Set2 methyltransferase provides evidence for transcription-dependent and -independent dynamics of H3K36 methylation. Genome Res. 2020;30:1605-1617 pubmed publisher
product information
Catalog Number :
122003
Product Name :
pNIA-CEN-mVenus-FLAG-LANS1-Set2
article :
doi10.1101/gr.264283.120
id28197262
pubmed_id33020206
bacterial resistance :
Ampicillin
cloning :
backbonepNIA-CEN
backbone_mutation
backbone_origin
backbone_size8039
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
Synthetic Biology
growth notes :
Please note: the Addgene ID for this plasmid is incorrectly listed in the publication as 122002. Addgene plasmid 122003 is mVenus-Flag-LANS-Set2 and Addgene plasmid 122002 is Flag-LANS-Set2.
growth strain :
mVenus-FLAG-LANS-Set2: mVenus- and FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast
origin :
37
pi :
alt_names
mVenus-FLAG-LANS-Set2
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterADH1
sequencing_primer_3GGGACCTAGACTTCAGGTTGTCTAACTCC
sequencing_primer_5GTCATTGTTCTCGTTCCCTTTCTTCCTTG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYJL168C, EZL1, KMT3
geneSET2
id853271
genbank_ids
mutationSet2 point mutations K538G, K539S, R549G, K550S, K551G
namemVenus-FLAG-LANS1-Set2
shRNA_sequence
size3492
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on insert
tagmVenus-FLAG
resistance markers :
1351
tags :
High Copy
terms :
LEU2
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA