This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
hGli3 NTOTAL in AINGpCAGGS
catalog :
121973
citations: 1
Reference
Ulloa F, Itasaki N, Briscoe J. Inhibitory Gli3 activity negatively regulates Wnt/beta-catenin signaling. Curr Biol. 2007;17:545-50 pubmed
product information
Catalog Number :
121973
Product Name :
hGli3 NTOTAL in AINGpCAGGS
article :
doi10.1016/j.cub.2007.01.062
id28197379
pubmed_id17331723
bacterial resistance :
Ampicillin
cloning :
backboneLinker A IRES NLS GFP pCAGGS
backbone_mutation
backbone_originhome made using pCAGGS
backbone_size6200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
chick electroporation
growth notes :
The GLI3 insert contains an S1131G difference compared to NM_000168.6 that does not affect function.
growth strain :
medium Gli3 transcriptional repressor
origin :
37
pi :
alt_names
Gli3R
cloning
clone_methodRestriction Enzyme
cloning_site_3SacI
cloning_site_5ClaI
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACLS, GCPS, GLI3-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV
geneGLI3
id2737
genbank_ids
mutationC terminus deletion from zinc finger domain
namehGli3 NTOTAL
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tag5xmyc
locationC terminal on backbone
tagLinker A IRES NLS GFP pCAGGS
resistance markers :
1909
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA