This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
DN1-Gli3 in AINGpCAGGS
catalog :
121970
citations: 1
Reference
Stamataki D, Ulloa F, Tsoni S, Mynett A, Briscoe J. A gradient of Gli activity mediates graded Sonic Hedgehog signaling in the neural tube. Genes Dev. 2005;19:626-41 pubmed
product information
Catalog Number :
121970
Product Name :
DN1-Gli3 in AINGpCAGGS
article :
doi10.1101/gad.325905
id28197378
pubmed_id15741323
bacterial resistance :
Ampicillin
cloning :
backboneLinker A IRES NLS GFP pCAGGS
backbone_mutation
backbone_originhome made using pCAGGS
backbone_size6200
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Other
chick electroporation
growth notes :
The GLI3 insert contains an S1131G difference compared to NM_000168.6 that does not affect function.
growth strain :
medium Gli3 transcriptional activator
origin :
37
pi :
alt_names
Gli3A^MED
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5XhoI
promoter
sequencing_primer_3
sequencing_primer_5T7
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesACLS, GCPS, GLI3-190, GLI3FL, PAP-A, PAPA, PAPA1, PAPB, PHS, PPDIV
geneGLI3
id2737
genbank_ids
mutationN terminus deletion (nt1-864)
nameDN2Gli3
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tag5xmyc
resistance markers :
1909
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA