This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold
catalog :
121841
citations: 1
Reference
Haldeman J, Conway A, Arlotto M, Slentz D, Muoio D, Becker T, et al. Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Nucleic Acids Res. 2019;47:e23 pubmed publisher
product information
Catalog Number :
121841
Product Name :
JI501: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 gRNA scaffold
article :
doi10.1093/nar/gky1286
id28197237
pubmed_id30590691
bacterial resistance :
Kanamycin
cloning :
backbonepDONR221 P3-P2
backbone_mutation
backbone_originInvitrogen
backbone_size2555
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Synthetic Biology
Other
pMAGIC Gateway Entry Plasmid
growth notes :
Annealed oligonucleotides containing the protospacer motif can be ligated into BsaI digested plasmid. 3x HA tag + bGH polyA; hU6 promoter PCR'd from Addgene 61591. SaCas9 gRNA scaffold derived from PMID: 26721686
growth strain :
pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; empty hU6-driven SaCas9 gRNA scaffold for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to dCas9 w/ gRNA expression
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13R
sequencing_primer_5M13F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
name3x HA epitope tag-PolyA + hU6-SaCas9 gRNA scaffold
shRNA_sequence
size709
species
100
Synthetic
tags
resistance markers :
4311
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA