This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pA
catalog :
121800
citations: 1
Reference
Haldeman J, Conway A, Arlotto M, Slentz D, Muoio D, Becker T, et al. Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing. Nucleic Acids Res. 2019;47:e23 pubmed publisher
product information
Catalog Number :
121800
Product Name :
KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pA
article :
doi10.1093/nar/gky1286
id28197237
pubmed_id30590691
bacterial resistance :
Kanamycin
cloning :
backbonepDONR221 P3-P2
backbone_mutation
backbone_originInvitrogen
backbone_size2555
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Synthetic Biology
Other
pMVP Gateway Entry Plasmid
growth notes :
TETa sequence derived from Tet-On Advanced system (Clontech)
growth strain :
pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven GFP-P2A-TETa
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3M13R
sequencing_primer_5M13F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameHA epitope tag-polyA + CMV::eGFP-P2A-TETa-polyA
shRNA_sequence
size2936
species
100
Synthetic
tags
resistance markers :
4311
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA