This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSUC2::GNSI (NrsR)
catalog :
121537
citations: 1
Reference
Plemel R, Odorizzi G, Merz A. Genetically encoded multimode reporter of AP-3 traffic in budding yeast. Traffic. 2020;: pubmed publisher
product information
Catalog Number :
121537
Product Name :
pSUC2::GNSI (NrsR)
article :
doi10.1111/tra.12772
id28193445
pubmed_id33225520
bacterial resistance :
Ampicillin
cloning :
backbonepRS415
backbone_mutation
backbone_origin
backbone_size6021
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
bioRxiv pre-print 314286: Plate-based assay of AP-3 traffic in budding yeast Please visit https://www.biorxiv.org/content/10.1101/314286v2
growth strain :
This plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.
origin :
37
pi :
alt_names
cloning
entrez_gene
aliasesYJR048W
geneCYC1
id853507
genbank_ids
mutation
nameCYC1 Promoter
shRNA_sequence
size290
species
4932
Saccharomyces cerevisiae
tags
alt_names
GNS cassette
cloning
entrez_gene
genbank_ids
mutationM109I (Naturally occurring SNP)
nameNyv1 Cytosolic Domain (aa 6-230)
shRNA_sequence
size675
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on insert
tagmGFP5
alt_names
GNS cassette
cloning
entrez_gene
genbank_ids
mutation
nameSnc1 Transmembrane Domain (TMD)
shRNA_sequence
size60
species
4932
Saccharomyces cerevisiae
tags
alt_names
GSI cassette
cloning
entrez_gene
genbank_ids
mutationThe first 21 residues are deleted, removing the localization signal sequence
nameSUC2 CDS
shRNA_sequence
size1539
species
4932
Saccharomyces cerevisiae
tags
alt_names
GSI cassette
cloning
entrez_gene
genbank_ids
mutation
nameSUC2 terminator
shRNA_sequence
size459
species
tags
alt_names
NatMX6
cloning
entrez_gene
genbank_ids
mutation
nameNrsR
shRNA_sequence
size1105
species
32644
Other
Streptomyces noursei
tags
plasmid copy :
pGNS416, encoding GFP-Nyv1-Snc1TMD (GNS cassette) was a gift from F. Reggiori {Reggiori et al., 2000 PMID 11071903}. pHB4, encoding GSI (in earlier work called GSS) was a gift from E. Conibear {Burston et al., 2009, Addgene #53462}.
resistance markers :
3945
tags :
Low Copy
terms :
LEU2
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA