This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAAV-DIO-trkB.DN-mCherry
catalog :
121502
citations: 1
Reference
Guyon N, Zacharias L, van Lunteren J, Immenschuh J, Fuzik J, Martin A, et al. Adult trkB Signaling in Parvalbumin Interneurons is Essential to Prefrontal Network Dynamics. J Neurosci. 2021;41:3120-3141 pubmed publisher
product information
Catalog Number :
121502
Product Name :
pAAV-DIO-trkB.DN-mCherry
article :
doi10.1523/JNEUROSCI.1848-20.2021
id28197210
pubmed_id33593856
bacterial resistance :
Ampicillin
cloning :
backbonepAAV
backbone_mutation
backbone_originInvitrogen
backbone_size5591
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
AAV
Cre/Lox
growth strain :
Expresses trkB.T1 fused with mCherry in a cre dependent manner.
growth temp :
Please use Rec A- competent cells such as Stbl3 cells from Invitrogen for transformation.
origin :
37
pi :
alt_names
truncated version of tyrosine receptor kinase B
NTrk2 variant 2
cloning
clone_methodRestriction Enzyme
cloning_site_3NheI
cloning_site_5AscI
promoterEF1a
sequencing_primer_3GCAATAGCATGATACAAAGG
sequencing_primer_5ggccagcttggcacttgatg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRATTRKB1, TRKB1, Tkrb, trk-B, trkB
geneNtrk2
id25054
genbank_ids
mutation
nametrkB.T1
shRNA_sequence
size2076
species
10116
Rattus norvegicus
tags
locationC terminal on insert
tagmCherry
plasmid copy :
trkB.T1 is cloned from prattrkBT1 from Lino Tessarollo's group at National Cancer Insitute, NIH
resistance markers :
4253
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA