This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pTriEx-RhoA FLARE.sc Biosensor WT
catalog :
12150
citations: 15
Reference
Gupta S, Duszyc K, Verma S, Budnar S, Liang X, Gomez G, et al. Enhanced RhoA signalling stabilizes E-cadherin in migrating epithelial monolayers. J Cell Sci. 2021;134: pubmed publisher
Wu D, Harrison D, Szasz T, Yeh C, Shentu T, Meliton A, et al. Single-cell metabolic imaging reveals a SLC2A3-dependent glycolytic burst in motile endothelial cells. Nat Metab. 2021;3:714-727 pubmed publisher
Inaba H, Miao Q, Nakata T. Optogenetic control of small GTPases reveals RhoA mediates intracellular calcium signaling. J Biol Chem. 2021;:100290 pubmed publisher
Zhang J, Nguyen A, Miyamoto S, Heller Brown J, McCulloch A, Zhang J. Histamine-induced biphasic activation of RhoA allows for persistent RhoA signaling. PLoS Biol. 2020;18:e3000866 pubmed publisher
Horn A, Raavicharla S, Shah S, Cox D, Jaiswal J. Mitochondrial fragmentation enables localized signaling required for cell repair. J Cell Biol. 2020;219: pubmed publisher
Shen W, Kilander M, Bridi M, Frei J, Niescier R, Huang S, et al. Tomosyn regulates the small RhoA GTPase to control the dendritic stability of neurons and the surface expression of AMPA receptors. J Neurosci Res. 2020;98:1213-1231 pubmed publisher
Acharya B, Nestor Bergmann A, Liang X, Gupta S, Duszyc K, Gauquelin E, et al. A Mechanosensitive RhoA Pathway that Protects Epithelia against Acute Tensile Stress. Dev Cell. 2018;47:439-452.e6 pubmed publisher
Jensch A, Frey Y, Bitschar K, Weber P, Schmid S, Hausser A, et al. The tumor suppressor protein DLC1 maintains protein kinase D activity and Golgi secretory function. J Biol Chem. 2018;293:14407-14416 pubmed publisher
Eisler S, Curado F, Link G, Schulz S, Noack M, Steinke M, et al. A Rho signaling network links microtubules to PKD controlled carrier transport to focal adhesions. elife. 2018;7: pubmed publisher
Raya Sandino A, Castillo Kauil A, Domínguez Calderón A, Alarcón L, Flores Benitez D, Cuellar Perez F, et al. Zonula occludens-2 regulates Rho proteins activity and the development of epithelial cytoarchitecture and barrier function. Biochim Biophys Acta Mol Cell Res. 2017;1864:1714-1733 pubmed publisher
Zhang X, Adderley S, Breslin J. Activation of RhoA, but Not Rac1, Mediates Early Stages of S1P-Induced Endothelial Barrier Enhancement. PLoS ONE. 2016;11:e0155490 pubmed publisher
Sroka R, Van Lint J, Katz S, Schneider M, Kleger A, Paschke S, et al. Cortactin is a scaffolding platform for the E-cadherin adhesion complex and is regulated by protein kinase D1 phosphorylation. J Cell Sci. 2016;129:2416-29 pubmed publisher
Palandri A, Salvador V, Wojnacki J, Vivinetto A, Schnaar R, Lopez P. Myelin-associated glycoprotein modulates apoptosis of motoneurons during early postnatal development via NgR/p75(NTR) receptor-mediated activation of RhoA signaling pathways. Cell Death Dis. 2015;6:e1876 pubmed publisher
Gonzalez Billault C, Muñoz Llancao P, Henríquez D, Wojnacki J, Conde C, Caceres A. The role of small GTPases in neuronal morphogenesis and polarity. Cytoskeleton (Hoboken). 2012;69:464-85 pubmed publisher
Pertz O, Hodgson L, Klemke R, Hahn K. Spatiotemporal dynamics of RhoA activity in migrating cells. Nature. 2006;440:1069-72 pubmed
product information
Catalog Number :
12150
Product Name :
pTriEx-RhoA FLARE.sc Biosensor WT
article :
doi10.1038/nature04665
id665
pubmed_id16547516
bacterial resistance :
Ampicillin
cloning :
backbonepTriEx
backbone_mutation
backbone_originNovagen
backbone_size5000
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Bacterial Expression
Insect Expression
origin :
37
pi :
alt_names
6xHis-RBD-CFP-Linker-Citrine-RhoA WT
RhoA
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NcoI
promoter
sequencing_primer_3
sequencing_primer_5TriExUP
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesARH12, ARHA, EDFAOB, RHO12, RHOH12
geneRHOA
id387
genbank_ids
mutation
nameRhoA Biosensor
shRNA_sequence
size2400
species
9606
Homo sapiens
tags
locationN terminal on insert
tag6xHis
resistance markers :
185
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA